Objective: To investigate wound healing as well as fibroblasts and neutrophil numbers on a limited skin field which is previously exposed to combined Infrared and Electrical stimulation.
Methods: 20 male rabbits were randomly assigned to two groups each one of 10 animals. The experimental group was exposed to combine Infrared (IR) and Electrical Stimulation (ES) at dorsal area for three days respectively, in day four an incision was made in the center of exposed skin then sutured. A biopsies were taken at 24,48,72 hours, week and two weeks after surgery for counting of fibroblasts and neutrophil numbers as well as for histological observation. The same procedure was made for control group without exposing to radiation and stimulation.
Results: There was statistically significant difference in fibroblasts and neutrophil numbers between two groups (p<0.01). The number of these cells were higher in experimental group than control in all periods. Histological observation revealed good orientation of fibroblasts and collagen fibers in experimental group as well as excellent wound healing.
Conclusion: This study suggested that application of IR combined with ES on limited skin field have beneficial effects on fibroblasts and neutrophil numbers of a wound created in same exposed field with good wound healing.