Kirkuk Journal of Science

Kirkuk Journal of Science

Activity of Alanine aminopeptidase and its isoenzyms partially purified from the urine of chronic renal failure patients.

Document Type : Research Paper

Authors
1 College of Education for women - University of Tikrit
2 College of Education - University of Tikrit
Abstract
The study was performed on 50 urine specimens of patients with chronic renal failure ,in addition to the 92 healthy specimens as a control group. The results of the study revealed that Alanine aminopeptidase (AAP) activity of chronic renal failure patients urine shows a highly significant increase (p<0.0001) compared to healthy subjects. Alanine aminopeptidase was purified from urine patients with chronic renal failure and healthy subjects by gel filtration using sephadex G-50, and two isoenzyms of (AAP) I, II were separated from patients urine using anion exchanger (DEAE-sephdexA-50).
Keywords

Volume 3, Issue 2
Autumn 2008
Page 11-22

  • Receive Date 01 December 2008
  • Revise Date 20 December 2008
  • Accept Date 25 December 2008