Abstract
One hundred eighty three serum samples were collected from healthy persons living in villages around Kirkuk city and tested for the presence of brucella antibodies using slide agglutination test. The results showed that 123 sample gives positive results ranged between 1/20 to 1/320 (67.2%), in addition Coomb΄s test was used also to test the serum samples which give negative result with slide agglutination test show 16.3% chronic cases. The investigations indicate that the populations of these villages are highly susceptible to Brucella antigens, so the normality of antigen 1/160 suggested being the base line for diagnosis of Brucellosis in these rural areas.