This study determines the nature of microbial wound colonization in191 patients with wound infection attending Internal Lab of Teaching Hospital and Emergency Hospital in Erbil city during the period 1-January-2007 to31-July-2007.A total of 241 bacterial isolates were identified after culturing the swabs on different culture media. The results indicated that the most frequent isolates were Staphylococcus aureus (32.78%), Pseudomonas aeruginosa (24.90%) , Escherichia coli(14.94%), Enterobacter spp. (9.96%), Proteus mirabilis (8.71%), Klebsiella pneumoniae (6.64%) ,Klebsiella oxytoca (1.24%) and Citrobacter freundii (0.83%). Most of the isolates showed high level resistance to commonly available antibiotic. The present study also undertaken to assess the antimicrobial effect of aqueous,methanol and ethanol extracts of Quercus infectoria on the isolated bacterial species.The minimum inhibitory concentration (MIC) of ethanolic extract on Staphylococcus aureus was 3.125 mg/ml and that effect on Escherichia coli,Klebsiella pneumoniae and Citrobacte freundii were 6.25 mg/ml while the aqueous, methanolic and ethanolic extracts had effect at 25.0,12.5 and 6.25 mg/ml respectively on Pseudomonas aeruginosa Proteus mirabilis and Klebsiella pneumonia respectively.