The study included isolating and diagnosing Pseudomonas Aerugi-
nosa bacteria from different clinical samples from hospitals and health centers: 10 wound samples, 15 burn samples, 12 ear samples (otitis media), 11 urine samples and 9 sputum samples. These samples were diagnosed using cultural and biochemical features and confirmed by using the API 20E system test. The results showed that the isolation rate of Pseudomonas Aeruginosa bacteria from burns was (26.3%) and from ears was (21%). The resistance of Pseudomonas Aeruginosa isolates to 6 types of antibiotics was tested. The highest resistance rate (29.8%) was observed against Cefazoline and Carbenicillin, followed by resistance to Cefotaxime (17.5%), then to Gentamicin (14%), followed by Ciprofloxacin and Amikacin, (5.2%) and (3.5%), respectively. The results demonstrated that 18(31.5%) of Pseudomonas Aeruginosa isolates produced the pyocyanin pigment on Nutrient agar media and 9(15.7%) of them produced the pigment on MacConkey agar, while 15(26.3%) isolates produced the pigment on Blood agar. The burn isolates were shown to produce the highest pyocyanin pigments, and nutrient agar was the highest media that produced pyocyanin.