The dysfunction of kidney dysfunctions is one of the most common diseases among people on a global scale. and the renal failure disease were subdivided into five stages, which include decrease glomerular filtration rate 60 ml/ min, levels in serum. The aim of the current study was to estimate the concentrations of erythropoietin, hepcidin and albumin in people with chronic renal failure. The current study included 75 Patients of both gender, ages (40-70). They suffer from renal failure at different stages which has been classified into five stages and each stage include 15 sample as well as, 15healthy people as a control group. the results showed a significant decrease (P≤0.01) in the concentration of erythropoietin in chronic kidney disease (CKD) patients compared with the control group, as the concentration of erythropoietin began to decrease gradually during the stages of renal failure due to the decline in renal function in CKD patients as a result of decreased filtration and secretion of this hormone. The results showed a significant increase (P≤0.01) in the concentration of hepcidin in CKD patients, except for the first stage, in which a significant difference was not observed when compared with the control group. Finally, the results also showed a significant (P≤0.01) decrease in albumin levels in patients with renal failure compared to the control group. Based on the results of the current study, both erythropoietin and hepcidin can be used as indicators of renal failure. Also, albumin is one of the most important criteria for detecting kidney function.