The optical properties of agarose thin films, such as absorption, absorption coefficient, and extinction coefficient, are presented in this study. To obtain a 2% wt/v concentration of agarose, 0.2 mg of agarose was dissolved in 10 mL of water, and the mixture was then heated in the microwave for 30 second until boiling. In the current investigation, three types of water were used, including distilled water labeled as W1 (pH = 7.5 and TDS = 18 ppm), Zamzam water labeled as W2 (pH = 8 and TDS = 339 ppm), and so forth well water with the designation W3 (pH =8.6 and TDS = 1440 ppm). Total dissolving salts (TDS) and pH vary across the aforementioned types of water. The agarose thin film was irradiated using a (Nd:YAG) laser with three different wavelengths, including (532, 1064, and 1320) nm. Investigations into the impacts of changing the laser's wavelength on the optical characteristics of agarose thin films were conducted.