Spectrophotometric Determination of Methyldopa in Pure and Pharmaceutical Preparations by the Oxidative Coupling Reaction with 1, 5-Diaminonaphthalene in the Presence of Ammonium Ceric (IV) Nitrate

Oxidative coupling reactions are one of the significant methods used in the estimation of certain drugs in pharmaceutical formulations. This study describes the development of a fast and sensitive spectroscopic method considering the determination of trace amounts of methyldopa in an acidic aqueous solution.The adopted method centered on oxidative coupling due to the drug Methyldopa with the reagent 1, 5-diaminonaphthalene in the presence of ceric (IV) ammonium nitrate as oxidizing agent to form a stable blue-colored product, giving the highest absorption at 730 nm. The product was appeared to agree Beer's law within the limits of (5.4 - 39.6) μg /ml, and a value of molar absorptivity equal to 4.79471 ×104 L/ mol. cm. Sandell’s index, 0.0044 μg/ cm2, Relative standard deviation was in the range (0.5942 - 0.9687) %, whereas recovery average and determination factor were 99.908% and 0.9998. The proposed method has been successfully applied in the determination of Methyldopa in its pharmaceutical preparations.

Keywords:

Methyldopa

Spectrophotometric

Oxidative Coupling

Pharmaceutical

Main Subjects:

Chemistry

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[1] and J. E. Hoover A. Osol, J.T. Anderson. Remington’s pharmaceutical sciences: a treatise on the theory and practice of pharmaceutical sciences, with essential information about pharmaceutical and medicinal agents. Lippincott Williams Wilkins, 1st edition, 2006.

[2] Afsaneh L Sanati and Farnoush Faridbod. Electrochemical determination of methyldopa by graphene quantum dot/1-butyl-3-methylimidazolium hexafluoro phosphate nanocomposite electrode. Int. J. Electrochem. Sci, 12(9):7997–8005, 2017.

[3] L.E. Limbird P.B. Molinoff A.G. Gilman, J.G. Hardman and R.W. Ruddon. The pharmaceutical basis of therapeutics.

[4] C.R. Craig and R. E. Stitzel. Lmodern pharmacology with clinical applications.

[5] Safwan Ashour, Mahmoud Bahbouh, and Mouhammed Khateeb. A novel use of oxidative coupling reactions for determination of some statins (cholesterol-lowering drugs) in pharmaceutical formulations. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 78(3):913–917, 2011.

[6] Hongxiu Dai, Duo Chen, Yuan Li, Pengfei Cao, Nan Wang, and Meng Lin. Voltammetric sensing of dopamine based on a nanoneedle array consisting of nico2s4 hollow core-shells on a nickel foam. Microchimica Acta, 185(3):1–7, 2018.

[7] Paulo Roberto da Silva Ribeiro and Reginaria Morais ´ Duarte. Development and validation of a simple spectrophotometric method for the determination of methyldopa in both bulk and marketed dosage formulations. Brazilian Journal of Pharmaceutical Sciences, 50:573– 582, 2014.

[8] Samy Emara, Tsutomu Masujima, Walaa Zarad, Maha Kamal, Marwa Fouad, and Ramzia El-Bagary. An ecofriendly direct injection hplc method for methyldopa determination in serum by mixed-mode chromatography using a single protein-coated column. Journal of chromatographic science, 53(8):1353–1360, 2015.

[9] Xinhua Lin Jinghua Cheng Liying Huang Hong Yao, Yuan Yuan Sun. Flow-injection chemiluminescence determination of catecholamines based on their enhancing effects on the luminol-potassium periodate system. Luminescence: The journal of Biological and Chemical Luminescence, 21(2):112–7, 2006.

[10] Somayeh Tajik, Hadi Beitollahi, and Pourya Biparva. Methyldopa electrochemical sensor based on a glassy carbon electrode modified with cu/tio2 nanocomposite. Journal of the Serbian Chemical Society, 83(7-8):863– 874, 2018.

[11] Afsaneh L Sanati and Farnoush Faridbod. Electrochemical determination of methyldopa by graphene quantum dot/1-butyl-3-methylimidazolium hexafluoro phosphate nanocomposite electrode. Int. J. Electrochem. Sci, 12(9):7997–8005, 2017.

[12] Sylvain Bernes, Modesto Rodr ` ´ıguez Pastrana, Enrique Huerta Sanchez, and Ren ´ e Guti ´ errez P ´ erez. 1, 5- ´ diaminonaphthalene. Acta Crystallographica Section E: Structure Reports Online, 60(1):o45–o47, 2004.

[13] A.K. Al-Yasir. The effect of some biocontrol agents on some biological.aspects of home fly musca domestica l. (diptera: Muscidae). The Egyptian Journal of Hospital Medicine, 42:33–48, 2011.

[14] Themistocles P Hadjiioannou, Gary D Christian, Michael A Koupparis, and Panayotis E Macheras. Quantitative calculations in pharmaceutical practice and research. VCH New York, 1993.

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