The study investigates the effects of pomegranate peel extracts on some virulence factors such as the production of some enzymes produced by Proteus spp isolated from different clinical samples. Among 310 different clinical samples, 48 isolates of proteus were obtained, 14 isolates (29.16%) were obtained from urine, 15 isolates (31.25%) were obtained from stool, 7 isolates (14.58%) from ear swabs, 4 isolates (8.33%) were obtained from wounds, 5 isolates (10.41% (from burns and 3 isolates) 6.25%) of the high cervical. The study showed that from 48 isolates of Proteus spp 25 isolates (52.08 %) identified as  P.mirabilis, 15 isolates (31.25% )of P. penerri, while 8 isolates of P. vulgaris were obtained with a percentage of (16.66%) and it was found that 18 isolates (72%) of P.mirabilis isolates were positive for the production of beta-lactamase enzyme, 24 isolates (96%) are positive for proteases and 14 isolates (56%) produced lipase, while 13 isolates from P. penerri (86.66%) produced beta-lactamases and proteases and 10 isolates (66.66%) produced lipase, also found that 5 isolates (62.5%) of P. vulgaris produced Beta-lactamase and all the isolates (100%) produced protease while 7 isolates (87.5%) produced lipase. It was found that the hot aqueous extract of pomegranate peels inhibited the production of beta-lactamase enzyme in 4 out of 7 isolates that produced this enzyme, while all tested isolates did not produce lipase by using extracts of pomegranate peel extracts. The isolates lost their ability to produce protease enzyme, except for two isolates that were not affected by extracts of pomegranate peel fruits.