A total of 181 blood samples were collected from patients that suspected have been infected by septicemia from those how had burns infection or underwent surgery operation in the period of study that's extended from 1st January 2017 to 30th April 2018. Gram-positive Staphylococci constituted 28 isolates (50%) in total of 56 isolated isolates from 37 samples (20.44%), coagulase-negative staphylococci (CONS) constituted 17 isolates (60.7%) followed by coagulase-positive staphylococci which represented by Staphylococcus aureus 11(39.3%) from total of Gram-positive Staphylococci isolates, while obtained only one isolate of Leuconostoc mesenteroides. Pseudomonas aeruginosa occupied the highest ratio 29.6% in 8 isolates from total gram-negative 27 isolates (48.2%) followed by Escherichia coli and Enterobacter.spp in 6 isolates, Klebsiella pneumoniae 3 isolates, Acinetobacter baumannii in 2 isolates, while obtained only one isolate from Rautella terigena and Proteus mirabilis. This study is included also sensitivity test against sepsis bacterial pathogens by different type of antibiotics, bacterial species varied in resistance toward used antibiotics, where the all isolates are resistance against ampicillin, in the same ratio are sensitive toward tigeycycline except P. aeruginosa, while all isolates are sensitive toward Tecoplanin and linezolid in ratio 100%. 100.%