Purification, Estimation of Indole Acetic Acid of the Yeasts Kodamaea Ohmeri –AR1and Rhodotorula Mucilaginosa –AR and Determination of the Optimium Environmental Conditions for Its Production

Sulyman, Essam D.; Thanon, Arwa SH.

doi:10.32894/kujss.2019.14.4.6

Purification, Estimation of Indole Acetic Acid of the Yeasts Kodamaea Ohmeri –AR1and Rhodotorula Mucilaginosa –AR and Determination of the Optimium Environmental Conditions for Its Production

Document Type : Research Paper

Authors

Department of Biology, College of Science, University of Mosul, Mosul, Iraq.

10.32894/kujss.2019.14.4.6

Abstract

The results indicated that (5) isolates only of yeasts of them have proved their ability for IAA production with different concentrations. Best yeast isolates where chosen for IAA production the yeast; K. ohmeri –AR1and Rh.mucilaginosa –AR the best isolates to produced 109 and 107 g/ml for each of them respectively, when grown on YPG media at 28℃ for 5 days. Spectrophotometer were used for this purpose. The highest production of IAA were noticed when incubated for 7 days and reached 222.2 and 217.6 g/ml respectively. Results also declared that glucose was the best carbon source the production of IAA for K.ohmeri –AR1and manitol for Rh.mucilaginosa –AR and reached 119.4 and 58.3 g/ml respectively. While trypton was the better source of nitrogen and gave 149.0 g/ml for K.ohmeri –AR1and pepton for Rh.mucilaginosa –AR with 68.4 g/ml. Result also showed that pH level played an important role on the production of IAA where the high output was discovered at pH 6 for the growth of K.ohmeri –AR1gave 267.9 and pH 7 produced 246.5 g/ml for Rh.mucilaginosa-AR . The highest concentration of IAA production was noticed when tryptophan was used at 0.3% for growing of Rh.mucilaginosa –AR and 0.4% for K.ohmeri –AR1 where hormone production was about 191.3 and 202.8 g/ml for each of them respectively. The estimation of IAA was also done by HPLC which gave 96 g/ml of IAA for K.ohmeri –AR1and 44 g/ml for Rh.mucilaginosa-AR . Rh.mucilaginosa-AR .

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