Hypericum triquetrifolium is an herbaceous perennial plant, numerous of the bioactive compound are present in it that is responsible for several biological functions. Aim of this research the protective effect of aqueous extract of Hypericum triquetrifolium on some biochemical parameters and histopathological study in hyperlipidemic male rats. Thirty-six male albino rats were divided into six groups, control group (fed with standard pellet), hyperlipidemia group , three ,four and five groups hyperlipidemic rats treated with aqueous extract of Hypericum triquetrifolium in different dosage (1000,2000,3000) mg/kg body weight, respectively, and last group hyperlipidemic rats treated with rosuvastatin drug 10 mg/kg for 60 days . The results showed that the body weight, serum cholesterol, triglyceride, low-density lipoprotein, very low-density lipoprotein, cholesterol/high-density lipoprotein ratio, and hepatic enzymes increased significantly (p˂0.05) in hyperlipidemia group when compared with control group, but Hypericum triquetrifolium have ability to reduction the level of body weight, serum cholesterol, triglyceride, low-density lipoprotein, very low-density lipoprotein, cholesterol/high-density lipoprotein ratio, as well as the low dose of H. triquetrifolium nonsignificantly (p>0.05), prevent to increase liver enzymes, conversely the level of liver enzymes in rosuvastatin group treatment increased when compared with other treated and untreated groups. Noticeable damage of histology in testis showed degeneration in the germinal layer and fat change in liver of hyperlipidemia and rosuvastatin group, the low dose of H. triquetrifolium has beneficial effect on sperm parameters and histology of testis and liver. The quantity of aqueous extract of Hypericum triquetrifolium and antioxidant power of this plant have an essential role in their activity. Hypericum triquetrifolium .