Effect of Sodium Azide on Urease and Biofilm Formation and its Relationship to Swarming Phenomenon in Proteus Mirabilis

Al-Zaidi, Omar Sinan Sadiq; Abd Alhadee, Luma

doi:10.32894/kujss.2018.13.4.17

Effect of Sodium Azide on Urease and Biofilm Formation and its Relationship to Swarming Phenomenon in Proteus Mirabilis

Document Type : Research Paper

Authors

Omar Sinan Sadiq Al-Zaidi

Luma Abd Alhadee

Department of Biology, College of Education pure science/ Ibn Al. Haitham,University of Baghdad, Baghdad, Iraq.

10.32894/kujss.2018.13.4.17

Abstract

The current study was carried out in order to detect the effect of sodium azide on Urease production and biofilm formation in P.mirabilis. Twenty one isolates of Proteus were collected from different clinical and animals samples for the period from 15th October 2017 to 1st November 2017 and identification depending on microscopic characterization, biochemical tests and conformed by Vitec-2 compact system, its turns out that only 18 isolates belong to the specie P.mirabilis.All P.mirabilis isolates 100% were have the ability to produce urease enzyme.
All P.mirabilis 100% un able to form biofilm on Congo red agar while 100% were strongly able to form biofilm on microtiter plate. The addition of Sodium azide shown an inhibition ratio in biofilm formation depending on the concentration and source of isolate also shows inhibition ability in urease enzyme production in both swimmer cells and swarmer cells of P.mirabilis depending on the source of isolate and concentration.
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Keywords

urease

biofilm

Sodium azide

Swarming

Proteus mirabilis

Subjects