Effect of Using Different Compensation of Growth Regulators on Hypocotyls Callus Intiation and Differentiate of Medicago Sativa

Nawaf Al-zaidy, Raghad; Ibraheem Khalil Al-Barhawi, Najwa; Najim Abdullah Al-niemi, Abdullah

doi:10.32894/kujss.2018.145760

Effect of Using Different Compensation of Growth Regulators on Hypocotyls Callus Intiation and Differentiate of Medicago Sativa

Authors

Raghad Nawaf Al-zaidy

Najwa Ibraheem Khalil Al-Barhawi

Abdullah Najim Abdullah Al-niemi

10.32894/kujss.2018.145760

Abstract

The effect of some growth regulators on intiation ,grwth and differtiate of Alfalfa callus was studied .The search results showed a distinction MS media supplemented with growth regulators such as Kin ( 0.1 mgl) +2,4-D( 1.0 mg l) were distinguished in promoting callus formation from leaves and roots of Medicago sativa plants at ratio 90 and 100% successively. On the other hand the medium Kin (2.0mgL) +2,4-D (2.0mgL) formed callus from hypocotyl stem of seedlings in addition to formation of regeneration branches at number reached to 24 and of ratio 20% in one period.

Keywords

Growth Regulators

Medicago sativa

callus intiation and differentiate