Abstract
The effect of some growth regulators on intiation ,grwth and differtiate of Alfalfa callus was studied .The search results showed a distinction MS media supplemented with growth regulators such as Kin ( 0.1 mgl) +2,4-D( 1.0 mg l) were distinguished in promoting callus formation from leaves and roots of Medicago sativa plants at ratio 90 and 100% successively. On the other hand the medium Kin (2.0mgL) +2,4-D (2.0mgL) formed callus from hypocotyl stem of seedlings in addition to formation of regeneration branches at number reached to 24 and of ratio 20% in one period.