Hepatitis B infection is a worldwide healthcare problem, especially in developing areas. The current study was to evaluate the alterations in different biochemical parameters including paraoxonase (PON), 5′-Nucleotidase (5NT), total bilirubin, direct bilirubin, indirect bilirubin, Prothrombin time (PT), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), Total serum protein (TSP), albumin (Alb), Superoxide dismutase (SOD) and ɣ-glutamyl transpeptidase (GGT) in the serum samples of the viral hepatitis patients (n=100) compared with healthy controls(n=100). This study shown that there were significant increase (p<0.05) in the TSB, D. Bilirubin, In. Bilirubin, AST, ALT, PT, GGT, 5-NT, and ALP in viral hepatitis patients compared to their respective normal controls and there were significant decrease (p<0.05) in the serum (PON, SOD) activities, TSP and albumin concentration. It can be concluded that PON, 5-NT, SOD, ALT, AST, ALP and GGT may be specific method for making a diagnosis of viral hepatitis and also in distinguishing it with other kinds of hepatitis.