The aim of this study was to investigate person carries bacteria that cause typhoid fever (Salmonella typhi) in patients with cholelithiasis. For this purpose 50 sample of gallbladder tissue and 21sample only of bile salts(available only)collectedfrom the same patientswith cholelithiasis who subject to surgical removal in Azadi Teaching Hospital and Kirkuk General Hospital , in period from September 2016 to March 2017. The result showed that there was three out of 50 patients with gallstones were carried the bacteria which causes typhoid fever and this give ratio 6%. Females they was more than male in carried the pathogenic bacteria that give ratio 6.89% and 4.67% for females and males, respectively. Our study shows an association between presence stone in gallbladder and the presence of bacteria caused typhoid fever .