In this paper to study the spread and the distribution of the characteristic of resisting the medications of the Escherichia coli isolated from various sources. Therefore, Fifteen bacterial isolates were isolated and diagnosed for the E.coli (five isolates from the hospitals, five isolates from the wastewater and five isolates from clinical samples). The culture characteristics and biochemistry tests was certified in isolating and diagnosing these isolates.
The results of the tests of sensitivity against the antibiotics showed a variation in the distribution and the spread of the resistance against these antibiotics whether in the isolates from one source or within the different isolates sources for both the E.coli. Moreover, most of the isolates showed the multiple resistance against the antibiotics and the feature of resisting the Amoxicillin, Ampicillin and Erythromycin antibiotics was the most common amongst the isolates studies.
The results of the conjugation experiments showed that the bacterial conjugation it is possible to take place between the E. coli isolate and the standard Salmonella strain. When using the E. coli as a donor strain the conjugation took place with a frequency of (0.11× 10-4), and that the group of resistance against the antibiotics transferred to the conjugative isolate