Abstract
This study was conducted in order to identify virulence factors of the bacteria possessed
by the bacteria Burkholderia pseudomallie, which isolated from lobbies and lounges Azadi
Teaching Hospital, (200) sample were collected from lounges of operations (General
surgeries - catheter operations - surgery (ENT) operations of eyes and women's obstetrics
surgery) and lounges (internists – surgical – emergency – burns and women's) from the
period of (December --2 014) to (April -2015), The isolated bacteria ratio (15.584%), Tested
a number of virulence factor of Burkholderia pseudomallei, which been tested included ( β-
Lactamase enzyme - Capsule – Gelatenase enzyme - protease enzyme – Leicithenase
enzyme - Lipase enzym - adhesion factor - the presence of Pili - biofilm - decomposition of
blood (Heamolysin) and The rate of bacterial isolates Burkholderia pseudomallei produced
this factors(33.333- 100- 50- 41.666- 41.666-41.666-100-75-100-66.666%) respectively, The
current study concluded that all the isolate at lest possess four virulence factor from the
studied factors.