Kirkuk University Journal-Scientific Studies

Abstract

Three hundred clinical sample were collected from patients admitted and comers to
(Azadi Teaching, Kirkuk General, kidsGeneral) hospitals, 140 sample from females with
percentage (46.6%) and 160 sample frommale (53.3%), and through it found that 24
samples with percentage (17.14%) represented positive for bacterial growth in females and
26 samples with(16.25%) represented positive bacterial growth of male cases. The ability of
Pseudo. aeruginosa to produce pyocyanin on various media were show that 43 clinical
isolates of Pseudo.aeruginosa (86%) produced pyocyanin on two media (Nutrient agar,
Muller hinton agar) and 15 (30%) produced the pigment on Macconky agar and 24 isolates
(48%) on blood agar. While the effect of pyocyanin pigment on the growth of different
pathogenic bacterial isolates the results showed that a gram positive bacteria such as
Staphylococcus aureus was more sensitive to the pyocyanin pigment than gram negative bacteria (E.coli, klebsiella pneumonia) when grown on Nutrient agar or Muller hinton
agar.