90 swabs taken from burn wounds of patients admitted to and attending burn unit in Azadi teaching hospital in Kirkuk from the period (14/12/2014 to 19/3/2015). The age of patients ranged from 11 months to 77 years the burn wounds infected in both sexes of patients, but females more than male (40 females, 27 male). The variable degrees of burn was from first to third degree was observed in the 90 patients. Some of the samples (26.86%) from revealed a mixed bacterial infection and 73.13% were single bacterial infection. Different gram positive and gram negative bacterial were isolated in different percentages as staph. aureus was isolated from 12.79% of cultures, staph. epidermidis isolated in 9.30%. While gram negative bacteria was isolated as follows (pseudomonas aeruginosa in 32.55%, klebsiella pneumoniae and Enterobacter cloacae in 17.44% for each, E.coli in 8.13% and proteus mirabilis in 2.32%). P. aeruginosa was the highest percentage of the all gram positive and gram negative bacteria isolates has been obtained 28 isolation percentage 32.5% from all 86 isolates. P. aeruginosa was isolated from single and mixed isolation (gram negative and gram positive) bacteria (E. cloacae, E.coli, K. Pneumoniae, P. mirabilis, S. aureus, S.spidermidis). Regarding the sensitivity of the P. aeruginosa from the cultures to different antibiotics , Twelve antibacterial agents were used including (Cefotaxime , Imipenem , Gentamicin , Amoxicillin , Auqmentin , Ampicillin , Ceftriaxone , Ceftazidime , Carbencillin , Amikacin , Ciprofloxacin , Tetracyclin ). Out of these antibiotics Pseudomonas aeruginosa were more resistant about 100% to (Augmentin , Amoxicillin , Ampicillin( , 93% to the (Ceftriaxone , Cefotaxime , Carbenicillin , Imipenem Gentamicin, Tetracyclin ) , 89% Ciprofloxacin, while it found less than (64-57)% Amikacin and Ceftazidime respectively. When comparing multiple antibiotic resistance among bacteria isolated from the mixed and individual infections there was a difference in the results, where the proportion of resistant mixed infections for (12)
antibiotic was rates of 27.7% while the resistance of individual infection ratio Pseudomonas aeruginosa (12) antibiotic at 23-52 %.