The present study is aimed to evaluate the role of some markers in the diagnosis of sepsis among SIRS (systemic inflammatory response syndrome) patients. The study included 180 patients with SIRS criteria within 24 hour of admission. They were admitted to different hospitals in Mosul city for the period from January 2013 to January 2014. From each patient two blood samples were collected, one for blood culture and the other separated by centrifugation and kept frozen for ELISA tests of PCT, IL-1, TNF-α, IL-10 and IL-13. IL-1, IL-10, IL-13 and PCT were having a significant difference in mean between culture positive and negative cases. PCT was significantly highly elevated in patients with sepsis (P=0.004) with high sensitivity (93.7%) and specificity (90.1%). Many combinations of markers were used in the current study to find out the best combination of markers. The results of using combined sensitivity and specificity of PCT together with 2 anti-inflammatory cytokines (IL-10 and IL-13) were the best. They were an excellent markers with 100% sensitivity and the highest specificity.