Use of Some Plants Color as Alternative Staining of Bacteria

Hussein Kamel, Fouad; ChnarNajmaddin, And

doi:10.32894/kujss.2017.124934

Use of Some Plants Color as Alternative Staining of Bacteria

Authors

Fouad Hussein Kamel

and ChnarNajmaddin

10.32894/kujss.2017.124934

Abstract

Natural dyes from plants such as Stigma (Isatis sp.), Myrtle (Myrtussp.), Rosella (Hibiscussp.) and crust of Walnut (Juglanssp.) fruits were extracted by 95% ethyl alcohol or distilled water. Myrtle and Stigma weremixed dye with ratio 1:2, respectively, also Rosellaand crust of nut fruits were prepared with same ratio. Mixeddyes or stains prepared as alternative of Gram stainfor staining Gram negative and Gram positive bacteria. The results showedthat the wall of bacteria were stained. This is well comparable to Gram stain in respect to clarity, differentiation, and economic cost.

Keywords

plants extracts

gram stain

Stigma

Myrtle

Rosella