The present study aims to determine the prevalence of Toxoplasma gondii in cancer patients in Azadi Teaching Hospital a group of healthy individuals were used as a control . Several Serological methods such as Latex Agglutination Test LAT, Modified Agglutination Test2-ME, Rapid- Cassette Test and Enzyme Linked Immunosorbent Assay (ELISA) were used to investigate the anti-antibodies against Toxoplasma gondii type IgG and IgM.The study was conducted on184person, including 136 cancer patients and 48 normal people have been adopted as control samples. The Blood samples were collected from the beginning of February to the end of November 2014. The results showed that the rate of Toxoplasma infection in cancer patients was 47.06 % for latex agglutination test LAT and 28.70% by using the modified agglutination test 2-ME and 8.8% using the Rapid test, and ELISA test was 24.26%. The chronic infection rate was 18.38% and a rate 2.94% of was for acute infection and mixed infection(acute ,chronic) .