Kirkuk Journal of Science

Kirkuk Journal of Science

Use of some plants color as alternative stain in staining of bacteria

Authors
Abstract
Natural dyes from plants such as Stigma (Isatis sp.), Myrtle (Myrtussp.), Rosella (Hibiscussp.) and crust of Walnut (Juglanssp.) fruits were extracted by 95% ethyl alcohol or distilled water. Myrtle and Stigma weremixed dye with ratio 1:2, respectively, also Rosellaand crust of nut fruits were prepared with same ratio. Mixeddyes or stains prepared as alternative of Gram stainfor staining Gram negative and Gram positive bacteria. The results showedthat the wall of bacteria were stained. This is well comparable to Gram stain in respect to clarity, differentiation, and economic cost.
Keywords

Volume 11, Issue 3
Summer 2016
Page 248-253

  • Receive Date 01 September 2016
  • Revise Date 20 September 2016
  • Accept Date 25 September 2016