Mus musculus infection by Cryptosporidium parvum and treatment by Specific Immunoglobulin IgG after Extraction , purification and molecular weight determination

Abdulqader Salih Alaloosi, Thaer; A. Mohaemeed, Abdulkhaliq; M. Hassen, Omer

doi:10.32894/kujss.2016.124514

Mus musculus infection by Cryptosporidium parvum and treatment by Specific Immunoglobulin IgG after Extraction , purification and molecular weight determination

Authors

Thaer abdulqader salih alaloosi

Abdulkhaliq A. Mohaemeed

Omer M. Hassen

10.32894/kujss.2016.124514

Abstract

The study included examination of 3200 stool sample selected randomly for taken from patients attending gynecological and obstetrics hospital in alramadi city , for period 1-6-2001 to 30-6-2012 , C. parvum parasite discovered by two ways : Direct test using Zehil-Neelsen and ELISA . we showed that ELISA test was best from direct method by using Zehil-Neelsen modified stain . parasite purification and crashing and injection in rabbits for extraction IgG antibodies and using for infection parasite treatment after mice group infection and compared antibodies treated with chemical treatment and noticed Oocyst ejected with feces after examination some blood analysis and Examination IgG , IgM , IgA , C3 , C4 in blood f as a sign to inflammation get and infection remaining or finishing , show the study result clear increase in outside Oocyst number Known in positive control treatment , Wrong treatment rest others , was showing low significant in outside Oocyst number Known . Was treatments best for blood signs and outside Oocyst number Known and antibodies and complements it Spiramycin

Keywords

Cryptosporidium

ELISA

immunotherapy and chemotherapy