Kirkuk Journal of Science

Kirkuk Journal of Science

Constract molecular marker-based for identification some varieties of Iraqi date palm (Phoenix dactylifera L.) by PCR-RFLP.

Authors
Akeel H. Ali Al-Assie
Jaladet M. Salih. Jubrael
Abdullah S. Awad
10.32894/kujss.2015.124148
Abstract
This study was performed on ten varieties of Iraqi dates palm (Barhi, kiara Hamra, sugary, Zuhdi, Khstawi, Khadrawi, Tbrzel, Sayer (osta omran), Prem, Maktoum) to devise specific DNA finger print for a given class using three specialized primers within the SSR markers and then use three restricted enzymes within PCR-RFLP technique to reach the goal. one band was result from all varieties with molecular size 320bp for mpdIRD28 and 200bp for mpdIRD46 , mpdIRD01 locus respectively after performing the PCR reaction within SSR markers. The PCR-RFLP techniqae was used with three restricted enzymes HinfI, TaqI, EcoRI The results reveals the presence or absence of Restriction sites for hybrid alleles (haplotypes) in date palm.
Keywords
date
palm
Fingerprints
Haplotypes
PCR
RFLPs
Kirkuk Journal of Science
Volume 10, Issue 4
Autumn 2015
Page 134-148

  • Receive Date 01 December 2015
  • Revise Date 20 December 2015
  • Accept Date 25 December 2015