A total of 170 blood samples were collected from neonates(newborn) aged from 1-28 days showing clinical sings of septicemia. The period of study extended from December 2012 to May 2013. Bacterial agents(Gram positive) were isolated from (22.35 %) of samples. Coagulase Negative Staphylococcus were isolated with the highest percentage. Staphylococcus epidermidis was maximally isolated among bacteria (31.57 %) followed by Staphylococcus haemolyticus (26.31 %), Staphylococcus warneri (10.52 %), Staphylococcus saprophyticus (7.89 %) low percentage of Staphylococcus lentus (2.63 %) was obtained in this study. Staphylococcus aureus formed (21%). The proportion of sensitivity of the microorgamism to different antibiotics were presented. These antibiotics include Penicillin, Oxacillin, Gentamicin, Tobromycin, Erythromycin, Clindamycin, Vancomycin, Tetracyclin, Rifampin, Trimthoprim, Linezolid and Teicoplanin, The study revealed high sensitivity of microorganisms to most of antibiotic, except Staphylococcus aureus and Staphylococcus epidermidis that showed high resistance to Penicillin and Oxacillin.