Molecular Study of Sortase Enzyme in Streptococcus pyogenes Isolated From Patients With Tonsillitis in Kirkuk City

S. Ahmed Al-Jebori, Ibraheem; H. Saeed Al-Shwany, Zakao

doi:10.32894/kujss.2015.103487

Molecular Study of Sortase Enzyme in Streptococcus pyogenes Isolated From Patients With Tonsillitis in Kirkuk City

Authors

10.32894/kujss.2015.103487

Abstract

From a total of (300) throat swabs collected from patients suffering from tonsillitis whom they attend to some of Primary Health Care Centers in Kirkuk City during the period from November (2012) to May (2013). Patients age were from (2-60) years .Only (16) isolates of Strep. pyogenes were obtained. These isolates detected by standard bacteriological methods and by Polymerase Chain Reaction (PCR) technique by detecting the presence of the diagnostic gene (spy1258). Specific primers were used for the detection of the genes (srtA, srtB and srtC) which encode for sortase enzymes (SrtA, SrtB and SrtC) respectively. The results showed that all of the (16) isolates of Strep. pyogenes contain the gene (srtA) with a percentage of (100%), while only (7) isolates with a percentage of (43.75%) contain the gene (srtB), whereas (13) isolates with a percentage of (81.25%) contain the gene (srtC). Streptococcus pyogenes DNA amplification reveal that only (7) isolates has had all three enzymes.

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