Kirkuk University Journal-Scientific Studies

Abstract

The study was carried out in Kirkuk main blood bank for period from beginning of anuary 2013 to end of September 2013 to detect Toxoplasma gondii among blood donors .
A questionnaire form was arranged to get information from each blood donors including age, occupation and blood groups . Five ml of blood was collected from each blood donors, put in plain tube without anticoagulant, centrifuged and serum kept at -20ºc for serological tests. The level of Toxoplasma gondii was estimated using Latex and ELISA IgG and IgM . From 250 blood donors it was found that the rate of Toxoplasma was 25.2% the highest rate was among (30-39) years old while using ELISA-IgG the rate of positive cases was 19.6% with highest rate among (30-39) years; using ELISA-IgM the rate of positive cases was 8.8% the highest rate was among (40-49) years. In ELISA IgM and IgG the highest rate 11.6%. The highest rate was 34% among age groups (40-49)years. Regarding occupations the highest positivity rate was among private work with rate of seropositivity was 67%, 61%, 55%, 66% with Latex, IgG, IgM and IgG with IgM cases respectively . Regarding blood groups using Latex the highest rate was 44% among blood group A and in ELISA-IgG was 45% among blood group A also . While in ELISA –IgM and ELISA IgG+IgM the highest rate among blood group O (36% ,38%) respectively . It is concluded that, the distribution of Toxoplasma gondii was high among blood donors in main blood bank in Kirkuk . It is recommended to carry on further investigation for Toxoplasma gondii among blood donors in all part of the country .