Abstract
This research includes the development of spectrophotometric method for determination
of Tetracycline via oxidative coupling with the N,N-Diethyl-p-phenylene diamine reagent in
presence of oxidizing agentN-Bromosuccinimide in the alkaline medium oxidation time 15
min to form a colored-dye violet, which dissolved in water and showed the highest intensity
of absorption at λmax 552nm, the method has been obeyed Beer's Law in the concentration
range (10-32μg. ml-1), with molar absorptivity of 10579 L. mol-1. Cm-1, Sandel Index value
0.0454 μg. cm-2, the detection limited 0.329 μg. ml-1, with correlation coefficient 0.9942 ,
the relative standard deviation of the method does not exceed 0.302% the proposed method
was applied successfully for the determination of Tetracycline Hydrochloride on
pharmaceutical preparation by both the direct and standard addition methods for three
concentrations, the recovery ranged between 98.8-102.71%. the evaluations of results of
this method was a achieved applying t and F tests. The results showed that there is no
significant difference between the proposed and standard method, and thus the proposed method is of good validity for the determination Tetracycline in its pharmaceutical
preparations