This study was designed to determine the impact of Buttermilk on blood total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), very low density lipoprotein cholesterol (VLDL-C) and the efficacy of liver enzymes ALT, AST and ALP, healthy and hypercholesterol experimental Sprague-Dawley rats were used as a model for this study. Rats were divided into five groups (six rats each) they were, healthy control group, hypercholesterol control group and three hypercholesterol groups fed on Buttermilk, Almarai and activia. At the end of the experiment (28 days) results revealed a significant decrease (p<0.05) in total cholesterol of groups fed on Buttermilk (135.27, 120.09 and 117.41 mg/100ml respectively) compared with hypercholesterol control group (234.11 mg/100ml), triglycerides and LDL-C for rats fed on Buttermilk, Almarai and activia (99.38, 90.42, 89.27 and 63.23, 47.01, 43.88 mg/100ml respectively) compared with hypercholesterol control group (164.84 and 154.57) mg/100ml respectively, but an increase was noticed in HDL-C for same groups. as for the effectiveness of the enzyme ALT and AST, the largest significant decrease was in the group of rats fed on the activia as recorded (19.44 and 60.35) IU/L compared to the hypercholesterolemic group (41.23) IU /L. it was concluded that Buttermilk played a positive role to improve lipid profile in hypercholesterol rats..