University of Kirkuk Kirkuk Journal of Science 3005-4788 12 1 2017 03 28 Comparative diagnostic study of Klebsiella usingApi20E System and the device vitek2 and PCR دراسة تشخيصية مقارنة لجراثيم الكليبسيلا باستخدام نظام الـ Api20Eوجهاز الـvitek2 وجهاز الـ PCR 81 94 124848 10.32894/kujss.2017.124848 EN Dr .Salah Salman Zain AL- Abdeen AL- Talabany Bnar Mohammed Abd AL-Majed Journal Article 2017 03 01 The current Study included 273 samples colected from models of different clinical Samples (urine , Stool, blood , sputum, and swabs from the vagina ,wounds, ear and throat) which have been get on 30 (10.98% ) isolates of Klebsiella from total of various clinical samples. The rate of Klebsiella from urine samples was 26.6% of the total 30 samples and The isolation rate from stool and blood sampls was 16.6%, while in wound infection the rat was (13.3%). Klebsiella isolated from vaginal swabs with (10%), and with respect to ear infections the percentage of isolation that have emerged in this study are (3.33%) and isolated rate from the throat and sputum was (6.6%). After a Diagnosis results have shown the prevalence of K.pneumoniae with rate(23 with76.66%) followed by K.oxytoca rate (5with16.66%) and 2 K.terrigena isolates with( 6.66%) of the total 30 isolates. Twenty isolates were selected belonging to the species K.pneumoniae and K.oxytoca which diagnosed by Api20E for rediagnosing by(PCR and viteke2) , and the results of the diagnostic system Vitek2 showd that the Klebsiella which have been previously diagnosed byApi20E system (90%) K.pneumoniae and (10%) K.oxytoca while the genetic diagnosis results showed 80% of the isolates belonged to the type of the K.pneumoniae and 20% of isolates belonging to K.oxytoca اما في التشخيص الجيني فقد أبدت النتائج أن( 80% ) من العزلات كانت تابعة لنوع الـ K.pneumoniae و)20% ) من العزلات تابعة لنوع الـK.oxytoca. وتم الاستنتاج بان نتيجة التشخيص بنظام الـPCR كانت اقرب لنتائج نظام الـ APi20E وابعد من نتائج نظام الـVitek2 https://kujss.uokirkuk.edu.iq/article_124848_e76167029f993d688953d044beb66354.pdf