Keywords : ELISA


Detection and differentiation of Entamoeba histolytica and Entamoeba dispar by enzyme linked immuno sorbent assay

Hiro M. Obaid

Kirkuk University Journal-Scientific Studies, 2016, Volume 11, Issue 3, Pages 263-277
DOI: 10.32894/kujss.2016.124283

Amoebiasis is an important parasitic disease in human. The two species Entamoeba histolytica and Entamoeba dispar are morphologically identical but E. dispar is none pathogenic and is not associated with symptomatic amoebiasis. In this study, from July to December 2013, 397 (212 male, 185 female) stool samples from in and out patients in Kirkuk Azady Teaching Hospital were examined microscopically. 97 samples of them were positive for Entamoeba histolytica / dispar. Blood samples were collected from E. histolytica / dispar positive patients, and the sera were examined by ELISA for differentiating the two species and evaluating the IgG levels in their serum. The overall rate of E. histolytica / dispar detected microscopically was 24.4%, while when the positive samples examined by ELISA technique 89.7% of them were E. histolytica and 10.3% were considered to be E. disbar. The serum samples of 27.58% of the patients whom had E. histolytica were positive for IgG antibody. The most age group which was infected with E. histolytica / dispar in both sexes were 41-50 years with rates of 39.13, 34.6 % for each of males and females respectively. A significantly high frequency (62.9, 94.8 %) of E. histolytica / dispar positive samples were contained RBC and pus cells respectively for each cell type, and the highest rate (28.8, 39.1%) were for those samples contained three pluses respectively for each of RBC and pus cells. The conclusion is that there is a big necessity of a serology confirmatory test after microscopic detection of E. histolytica to avoid un necessary treatment.

Mus musculus infection by Cryptosporidium parvum and treatment by Specific Immunoglobulin IgG after Extraction , purification and molecular weight determination

Thaer abdulqader salih alaloosi; Abdulkhaliq A. Mohaemeed; Omer M. Hassen

Kirkuk University Journal-Scientific Studies, 2016, Volume 11, Issue 2, Pages 197-226
DOI: 10.32894/kujss.2016.124514

The study included examination of 3200 stool sample selected randomly for taken from patients attending gynecological and obstetrics hospital in alramadi city , for period 1-6-2001 to 30-6-2012 , C. parvum parasite discovered by two ways : Direct test using Zehil-Neelsen and ELISA . we showed that ELISA test was best from direct method by using Zehil-Neelsen modified stain . parasite purification and crashing and injection in rabbits for extraction IgG antibodies and using for infection parasite treatment after mice group infection and compared antibodies treated with chemical treatment and noticed Oocyst ejected with feces after examination some blood analysis and Examination IgG , IgM , IgA , C3 , C4 in blood f as a sign to inflammation get and infection remaining or finishing , show the study result clear increase in outside Oocyst number Known in positive control treatment , Wrong treatment rest others , was showing low significant in outside Oocyst number Known . Was treatments best for blood signs and outside Oocyst number Known and antibodies and complements it Spiramycin

Seroprevalence of Toxoplasmosis among blood donors in Kirkuk main blood bank.

Essa H. Al-hadedy; Rana H. Al-jubory; Mohammed A. kadir

Kirkuk University Journal-Scientific Studies, 2015, Volume 10, Issue 2, Pages 107-128
DOI: 10.32894/kujss.2015.103483

The study was carried out in Kirkuk main blood bank for period from beginning of anuary 2013 to end of September 2013 to detect Toxoplasma gondii among blood donors .
A questionnaire form was arranged to get information from each blood donors including age, occupation and blood groups . Five ml of blood was collected from each blood donors, put in plain tube without anticoagulant, centrifuged and serum kept at -20ºc for serological tests. The level of Toxoplasma gondii was estimated using Latex and ELISA IgG and IgM . From 250 blood donors it was found that the rate of Toxoplasma was 25.2% the highest rate was among (30-39) years old while using ELISA-IgG the rate of positive cases was 19.6% with highest rate among (30-39) years; using ELISA-IgM the rate of positive cases was 8.8% the highest rate was among (40-49) years. In ELISA IgM and IgG the highest rate 11.6%. The highest rate was 34% among age groups (40-49)years. Regarding occupations the highest positivity rate was among private work with rate of seropositivity was 67%, 61%, 55%, 66% with Latex, IgG, IgM and IgG with IgM cases respectively . Regarding blood groups using Latex the highest rate was 44% among blood group A and in ELISA-IgG was 45% among blood group A also . While in ELISA –IgM and ELISA IgG+IgM the highest rate among blood group O (36% ,38%) respectively . It is concluded that, the distribution of Toxoplasma gondii was high among blood donors in main blood bank in Kirkuk . It is recommended to carry on further investigation for Toxoplasma gondii among blood donors in all part of the country .

Serological Study of Human Cytomegalovirus (CMV) in Diabetic Patients in Kirkuk Governorate

Najat Abdul-Kadir Zaman

Kirkuk University Journal-Scientific Studies, 2015, Volume 10, Issue 1, Pages 58-70
DOI: 10.32894/kujss.2015.101355

The aim of this study was to know the relationship between the human cytomegalovirus and diabetes mellitus through detection of human cytomegalovirus (HCMV) infection in diabetic patients in Kirkuk governorate by screening of anti-human cytomegalovirus IgM and IgG antibodies in the serum of diabetic patients by using of Immunochromatography and detection of IgG by ELISA technique in blood samples . Blood samples collected from 82 diabetic women patients and 10 samples from healthy looking with out diabetic women .The results of this study by Immunochromatography method detected in 22 samples (26.82%) IgM only ,25(30.48%) samples IgG only and 2(2.43%) samples showed positive results for both IgM and IgG for HCMV . In contrast, the results of control group were negative for anti-HCMV IgM and IgG antibodies in both Immunochromatography and ELISA technique. ELISA technique was used to know the presence IgG of HCMV for detection the sensitivity and specificity of Immunochromatography compared with ELISA technique .
KeyWords : HCMV, Immunochromatography ,Diabetics,ELISA,

Efficacy of some laboratory methods in detecting giardia lamblia and cryptosporidium parvumin stool samples

Yahya J. Salman

Kirkuk University Journal-Scientific Studies, 2014, Volume 9, Issue 1, Pages 7-17
DOI: 10.32894/kujss.2014.89613

Background: Giardia duodenalis and Cryptosporidium parvum are the most prevalent intestinal parasites of human. It also infects a wide range of mammals .Two genotype of G.duodenalis (A and B) were commonly reported among humans with different frequency of distribution in different geographical locations. Methods :total of 434 stool samples were collected from peoples in kikrkuk city during October 2012toAugust 2013. Zinc sulphate flotation technique was applied on 226 positive stool, serological methods involve Giardia ELISA-corpo antigen, Direct fluorescent assay (DFA), and lateral immune-chromatography assay(Triage panel) .Extractions of Giardia genome DNA from stool samples were performed using QIAamp Stool Mini kit with a modified protocol. PCR- one step procedure was used to amplify a fragment of Giardia lamblia genome using k725 gene locus,(Mixture primers of human A and B assemblages) Results:The overall rate of intestinal parasitic infection was 52.07%,Giardia lamblia rate was 24.65%.Common isolated parasites were Cryptosporidium parvum ,Blastocystishominis, other intestinal protozoan parasites and nematode helminthes:7.60 & 5.76 %,7.37 and 6.68 % respectively. Sensitivity and specificity of PCR method and direct microscopy were higher than other four methods used for detecting giardiasis. Triage panel method exert high rate of giardiasis than revealing of Cryptosporidium and Entamoeba histolytica. From the application of five methods for Cryptosporidium diagnosis, DFA and modified Ziehl-Neelsen(MZN) methods show high sensitivity and specificity than other three methods. Application of PCR single step using mixture primers assemblages A and B, show high rate of sensitivity than other methods in detecting giardiasis.Amplified Giardia genome length extended from 220 to 750 bps with mean of 437.56 bps. Conclusions: PCR assay targeting K725 gene locus is a sensitive tool and discriminates mixed genotypes of G.lamblia. DFA and MZN are sensitive tools for detecting Cryptosporidium parvum in stool samples