Keywords : PCR


Interleukin-8 Level in Pregnant Women with Toxoplasmosis in Kirkuk City

Ahlam Ahmed Mahmood; Zainab Sulaiman Rezaig

Kirkuk University Journal-Scientific Studies, 2019, Volume 14, Issue 3, Pages 56-67
DOI: 10.32894/kujss.2019.14.3.6

A cross sectional study was carried out in Kirkuk city from 15th of June 2018 to 15th of December 2018. The study included 100 pregnant women and 50 healthy individuals. Their ages ranged between 17-45 years old who were admitted to Kirkuk general hospital.. Molecular tests for real-time PCR and serological testing for detection specific Toxoplasma gondii IgM and IgG and Interleukin-8 level by using ELISA technique was done for patients and control. The study showed that the highest rate of anti T. gondii IgM+ IgG- antibodies (10%) was recorded among pregnant women compared with 8% in the control group, while 22% of pregnant women were IgM+IgG+ compared 6.5% of the healthy control group. The study revealed that 40.91% of pregnant women with positive ELISA was positive by PCR compared with 0% of patients with negative ELISA results. The study showed that the highest rate of T. gondii infection (diagnosed by PCR) were recorded among pregnant women at age group 27-36 year (22.55%) and the lowest rate was within the age group 17-26 year. The highest mean level of IL-8 recorded PCR +ve groups, in pregnant women (79.2 ±53.2 ng/ml) compared with PCR –ve groups. There was a highly significant differences of IL-8 between pregnant women and the control group. The study showed that the highest mean level of IL-8 (77.61±60.4 ng/ml), in pregnant at 2nd trimester of pregnancy, followed by 3rd trimester. This study was concluded that a highly elevation of IL-8 level was correlated Toxoplasma infection in pregnant women and real time PCR is golden method in diagnosis of toxoplasmosis..

Comparative diagnostic study of Klebsiella usingApi20E System and the device vitek2 and PCR

Dr .Salah Salman Zain AL- Abdeen AL- Talabany; Bnar Mohammed Abd AL-Majed

Kirkuk University Journal-Scientific Studies, 2017, Volume 12, Issue 1, Pages 81-94
DOI: 10.32894/kujss.2017.124848

The current Study included 273 samples colected from models of different clinical Samples (urine , Stool, blood , sputum, and swabs from the vagina ,wounds, ear and throat) which have been get on 30 (10.98% ) isolates of Klebsiella from total of various clinical samples. The rate of Klebsiella from urine samples was 26.6% of the total 30 samples and The isolation rate from stool and blood sampls was 16.6%, while in wound infection the rat was (13.3%). Klebsiella isolated from vaginal swabs with (10%), and with respect to ear infections the percentage of isolation that have emerged in this study are (3.33%) and isolated rate from the throat and sputum was (6.6%).
After a Diagnosis results have shown the prevalence of K.pneumoniae with rate(23 with76.66%) followed by K.oxytoca rate (5with16.66%) and 2 K.terrigena isolates with( 6.66%) of the total 30 isolates.
Twenty isolates were selected belonging to the species K.pneumoniae and K.oxytoca which diagnosed by Api20E for rediagnosing by(PCR and viteke2) , and the results of the diagnostic system Vitek2 showd that the Klebsiella which have been previously diagnosed byApi20E system (90%) K.pneumoniae and (10%) K.oxytoca while the genetic diagnosis results showed 80% of the isolates belonged to the type of the K.pneumoniae and 20% of isolates belonging to K.oxytoca

Constract molecular marker-based for identification some varieties of Iraqi date palm (Phoenix dactylifera L.) by PCR-RFLP.

Akeel H. Ali Al-Assie; Jaladet M. Salih. Jubrael; Abdullah S. Awad

Kirkuk University Journal-Scientific Studies, 2015, Volume 10, Issue 4, Pages 134-148
DOI: 10.32894/kujss.2015.124148

This study was performed on ten varieties of Iraqi dates palm (Barhi, kiara Hamra, sugary, Zuhdi, Khstawi, Khadrawi, Tbrzel, Sayer (osta omran), Prem, Maktoum) to devise specific DNA finger print for a given class using three specialized primers within the SSR markers and then use three restricted enzymes within PCR-RFLP technique to reach the goal. one band was result from all varieties with molecular size 320bp for mpdIRD28 and 200bp for mpdIRD46 , mpdIRD01 locus respectively after performing the PCR reaction within SSR markers. The PCR-RFLP techniqae was used with three restricted enzymes HinfI, TaqI, EcoRI The results reveals the presence or absence of Restriction sites for hybrid alleles (haplotypes) in date palm.

Efficacy of some laboratory methods in detecting giardia lamblia and cryptosporidium parvumin stool samples

Yahya J. Salman

Kirkuk University Journal-Scientific Studies, 2014, Volume 9, Issue 1, Pages 7-17
DOI: 10.32894/kujss.2014.89613

Background: Giardia duodenalis and Cryptosporidium parvum are the most prevalent intestinal parasites of human. It also infects a wide range of mammals .Two genotype of G.duodenalis (A and B) were commonly reported among humans with different frequency of distribution in different geographical locations. Methods :total of 434 stool samples were collected from peoples in kikrkuk city during October 2012toAugust 2013. Zinc sulphate flotation technique was applied on 226 positive stool, serological methods involve Giardia ELISA-corpo antigen, Direct fluorescent assay (DFA), and lateral immune-chromatography assay(Triage panel) .Extractions of Giardia genome DNA from stool samples were performed using QIAamp Stool Mini kit with a modified protocol. PCR- one step procedure was used to amplify a fragment of Giardia lamblia genome using k725 gene locus,(Mixture primers of human A and B assemblages) Results:The overall rate of intestinal parasitic infection was 52.07%,Giardia lamblia rate was 24.65%.Common isolated parasites were Cryptosporidium parvum ,Blastocystishominis, other intestinal protozoan parasites and nematode helminthes:7.60 & 5.76 %,7.37 and 6.68 % respectively. Sensitivity and specificity of PCR method and direct microscopy were higher than other four methods used for detecting giardiasis. Triage panel method exert high rate of giardiasis than revealing of Cryptosporidium and Entamoeba histolytica. From the application of five methods for Cryptosporidium diagnosis, DFA and modified Ziehl-Neelsen(MZN) methods show high sensitivity and specificity than other three methods. Application of PCR single step using mixture primers assemblages A and B, show high rate of sensitivity than other methods in detecting giardiasis.Amplified Giardia genome length extended from 220 to 750 bps with mean of 437.56 bps. Conclusions: PCR assay targeting K725 gene locus is a sensitive tool and discriminates mixed genotypes of G.lamblia. DFA and MZN are sensitive tools for detecting Cryptosporidium parvum in stool samples