Keywords : pseudomonas aeruginosa

The frequency of Pseudomonas aeruginosa bacteria with some pathogenic bacteria in burns injuries and study their resistance to antibiotics

Mohamed B. Khorsheed; salah S. Zain Al Abdeen

Kirkuk University Journal-Scientific Studies, 2017, Volume 12, Issue 1, Pages 123-140
DOI: 10.32894/kujss.2017.124967

90 swabs taken from burn wounds of patients admitted to and attending burn unit in Azadi teaching hospital in Kirkuk from the period (14/12/2014 to 19/3/2015). The age of patients ranged from 11 months to 77 years the burn wounds infected in both sexes of patients, but females more than male (40 females, 27 male). The variable degrees of burn was from first to third degree was observed in the 90 patients. Some of the samples (26.86%) from revealed a mixed bacterial infection and 73.13% were single bacterial infection. Different gram positive and gram negative bacterial were isolated in different percentages as staph. aureus was isolated from 12.79% of cultures, staph. epidermidis isolated in 9.30%. While gram negative bacteria was isolated as follows (pseudomonas aeruginosa in 32.55%, klebsiella pneumoniae and Enterobacter cloacae in 17.44% for each, E.coli in 8.13% and proteus mirabilis in 2.32%). P. aeruginosa was the highest percentage of the all gram positive and gram negative bacteria isolates has been obtained 28 isolation percentage 32.5% from all 86 isolates. P. aeruginosa was isolated from single and mixed isolation (gram negative and gram positive) bacteria (E. cloacae, E.coli, K. Pneumoniae, P. mirabilis, S. aureus, S.spidermidis). Regarding the sensitivity of the P. aeruginosa from the cultures to different antibiotics , Twelve antibacterial agents were used including (Cefotaxime , Imipenem , Gentamicin , Amoxicillin , Auqmentin , Ampicillin , Ceftriaxone , Ceftazidime , Carbencillin , Amikacin , Ciprofloxacin , Tetracyclin ). Out of these antibiotics Pseudomonas aeruginosa were more resistant about 100% to (Augmentin , Amoxicillin , Ampicillin( , 93% to the (Ceftriaxone , Cefotaxime , Carbenicillin , Imipenem Gentamicin, Tetracyclin ) , 89% Ciprofloxacin, while it found less than (64-57)% Amikacin and Ceftazidime respectively. When comparing multiple antibiotic resistance among bacteria isolated from the mixed and individual infections there was a difference in the results, where the proportion of resistant mixed infections for (12)
antibiotic was rates of 27.7% while the resistance of individual infection ratio Pseudomonas aeruginosa (12) antibiotic at 23-52 %.

Isolate the bacteria Pseudomonas aeruginosa from different clinical samples and study the effect of pyocyanin pigments of some pathogenic bacterial

Tara Fakhraddin raheem- Al-Jaff; Salah. S. Zain al-abdeen; Najdat .B. Mahdi

Kirkuk University Journal-Scientific Studies, 2016, Volume 11, Issue 4, Pages 215-226
DOI: 10.32894/kujss.2016.131098

Three hundred clinical sample were collected from patients admitted and comers to
(Azadi Teaching, Kirkuk General, kidsGeneral) hospitals, 140 sample from females with
percentage (46.6%) and 160 sample frommale (53.3%), and through it found that 24
samples with percentage (17.14%) represented positive for bacterial growth in females and
26 samples with(16.25%) represented positive bacterial growth of male cases. The ability of
Pseudo. aeruginosa to produce pyocyanin on various media were show that 43 clinical
isolates of Pseudo.aeruginosa (86%) produced pyocyanin on two media (Nutrient agar,
Muller hinton agar) and 15 (30%) produced the pigment on Macconky agar and 24 isolates
(48%) on blood agar. While the effect of pyocyanin pigment on the growth of different
pathogenic bacterial isolates the results showed that a gram positive bacteria such as
Staphylococcus aureus was more sensitive to the pyocyanin pigment than gram negative bacteria (E.coli, klebsiella pneumonia) when grown on Nutrient agar or Muller hinton

Detection of some virulence factors in Pseudomonas aeruginosa associated with diarrhea in Kirkuk City

Siham Sh. AL-Salihi; Abbas Y. Hasan

Kirkuk University Journal-Scientific Studies, 2015, Volume 10, Issue 1, Pages 78-89
DOI: 10.32894/kujss.2015.102016

Background: studies have shown that Pseudomonas aeruginosa can be a potential cause of antibiotic associated diarrhea. Pathogenesis based on endotoxin, exotoxins, and enzymes. Objectives: detection of some virulence factors produced by Pseudomonas aeruginosa isolated from faecal specimens in Kirkuk, Iraq.
Methods: The study included identification of Pseudomonas aeruginosa isolated from stool samples of children suffering from diarrhea, 518 samples collected from Azadi teaching hospital and Pediatric hospital in Kirkuk city, from February 2012 to June 2013. Various virulence factors including beta-lactamase, protease, lipase, lecithinase, deoxyribonuclease, gelatin liquefaction, haemolysin, congo red binding, urease, presence of capsule and beta-lactamase production were determined for Pseudomonas aeruginosa isolates.
Results: 35 Pseudomonas aeruginosa isolates were identified and the isolation percentage was 8.1% (only 433/518 give positive culture result for different bacteria). The distribution of virulence factors was different among the test isolates. All the isolates produce beta-lactamase but have different ability for binding to Congo red, gelatin liquefaction, lipase, protease, lecithinase, deoxyribonuclease, urease, capsule and haemolysin production.
Conclusion: Based on the findings of present study Pseudomonas aeruginosa isolated from diarrheal cases at least produced two virulent factors

Antibiosis resistant of Pseudomonas aeruginosa isolated from different clinical specimens

Siham Sh. AL-Salihi; Braihan H. Hameed

Kirkuk University Journal-Scientific Studies, 2014, Volume 9, Issue 2, Pages 15-28
DOI: 10.32894/kujss.2014.93719

Objective: Detection of antibiotic sensitivity of Pseudomonas aeruginosa isolated from different clinical specimens in Kirkuk, Iraq.
Material and Methods: The present study included (3138) samples collected from different clinical specimens from outpatients and inpatients of both sexes with different ages who were attended Azadi Teaching Hospital/ Kirkuk during the period from October/2007 until May/ 2013.
Results: From a total of 3138 Specimens, 1485 gave positive cultured and 1653 Specimens showed no bacterial growth, 319/3138 (10.17%) isolates were identified as P. aeruginosa. These isolates were identified according to morphological, cultural and biochemical characteristics. Wound and ear swab were important source for P. aeruginosa and isolated more frequently in inpatients than outpatients. The rate of isolation in females 169/319 (52.97%) was higher than males 150/319 (47.01%). Antibiotic susceptibility test of these isolates was performed, and the results showed that all Pseudomonas isolates (100%) were resistant to ampicillin, cephradin and trimoxazole, followed by gentamycin (97.3%), Amoxicillin (97.3), cephalexin (92.3%), neomycin (91.4%), nalidixic acid (89%), nitrofurantoin (87.5%), tobramycin (87.5%) and ciprofloxacin (84%), and the resistance to amikacin was (75%).
Conclusions: P. aeruginosa is multiresistant isolated in a high frequency from wound swabs followed by ear swabs.